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The present 2-D pattern is a high resolution 2-D reference map for human fertile spermatozoal protein spots.

A total of 3872 different protein spots were identified from the reference map, an approximately 3-fold increase compared to the broad range pH 3-10 IPG strip (1306 spots). The overlapping spots were excluded, so that every spot was counted only once. The 2-D reference map with pH value from 3.5 to 9.0 was synthesized by using the ImageMaster analysis software. After 2-DE, several visually identical spots between the different pH range 2-D gel pairs were cut from the gels and confirmed by mass spectrometry and used as landmarks for computer analysis. In order to reveal more protein spots, immobilized pH gradient strips (24 cm) of broad range of pH 3-10 and the narrower range of pH 6-9, as well as different overlapping narrow range pH immobilized pH gradient (IPG) strips, including 3.5-4.5, 4.0-5.0, 4.5-5.5, 5.0-6.0 and 5.5-6.7, were used. To extend the analysis of the proteome of human spermatozoa and establish a 2-D gel electrophoresis (2-DE) reference map of human spermatozoal proteins in a pH range of 3.5-9.0. Li, Ling-Wei Fan, Li-Qing Zhu, Wen-Bing Nien, Hong-Chuan Sun, Bo-Lan Luo, Ke-Li Liao, Ting-Ting Tang, Le Lu, Guang-Xiu These data will support the genome annotation process and provide a basis on which to undertake comparative proteomic studies.Įstablishment of a high-resolution 2-D reference map of human spermatozoal proteins from 12 fertile sperm-bank donors. Seventeen hypothetical or conserved hypothetical ORFs were shown to code for true gene products. Using MALDI-TOF-MS we identified 205 spots representing 173 different proteins with a calculated average of 1.18 proteins/gene.

Here, we present a 2-D reference map for H. Herbaspirillum seropedicae is an endophytic diazotroph associated with economically important crops such as rice, sugarcane, and wheat. A two-dimensional proteome reference map of Herbaspirillum seropedicae proteins.Ĭhaves, Daniela Fojo Seixas Ferrer, PÃ©rcio Pereira de Souza, Emanuel Maltempi Gruz, Leonardo MagalhÃ£es Monteiro, Rose Adele de Oliveira Pedrosa, FÃ¡bio

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